Transnetyx accepts embryo samples for genotyping. Transnetyx real-time PCR technology is extremely sensitive; any contamination with excess fluid can cause undetermined results. Please follow the guidelines below to collect embryonic samples.

1. A limb or tail of ~3 – 5 mm length is the preferred embryo sample size.

• If the embryo is too small for the above, cut through the pelvic area to collect the full lower limbs and tail as a single piece. 

2. If a limb or tail is not possible or is too difficult to collect, use the following procedure:

• Start with a piece of embryonic tissue, such as the head or abdomen.

• Using a clean cotton swab (Fisher cat# 23—400-114, for example), press the swab into the well and rotate to absorb all the sample and fluid. Swabs can be provided, upon request.

• Take the swab out with the tissue stuck to the swab. If necessary, use a second swab to dry the well. A relatively dry sample is very important.

• Remove a limb-sized piece of tissue from the first swab with small forceps and place back into the plate well (you may use any clean, sterile 96-well plate for this initial drying step).

• Place the samples in the designated Transnetyx 96-well plate, and firmly press the rubber cap mat into the wells to ensure no sample contamination in transit.

• Embryo tissue may be shipped at room temperature in a Transnetyx 96-well plate as usual. No special shipping is required.